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Flow Cytometric Analysis of PFA-fixed HeLa cells. SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells using SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGF尾/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP-2 and BMP-4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF尾 and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF尾 signaling by interfering with TGF尾-mediated phosphorylation of other Smad family members.
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