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Smooth MuscleMyosin Heavy Chain (SM-MHC)(Leiomyosarcoma & Myoepithelial Cell Marker) Antibody [SM-M10]

In Stock
Catalog Number Formulation Size Price
4629-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4629-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4629-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Smooth MuscleMyosin Heavy Chain (SM-MHC)(Leiomyosarcoma & Myoepithelial Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human uterus stained with SM-MHC Mouse Monoclonal Antibody (SM-M10). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Smooth MuscleMyosin Heavy Chain (SM-MHC)(Leiomyosarcoma & Myoepithelial Cell Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified SM-MHC Mouse Monoclonal Antibody (SM-M10). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Cow, Human, Mouse
Research Areas
Cardiovascular, Developmental Biology, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm.
Gene Name
MYH11
Positive Control
Uterus or normal breast.
Immunogen
Crude human uterus extract
Alternate Names
Myosin-11, Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, AAT4; Myosin heavy chain 11 (MYH11); Myosin heavy chain 11 smooth muscle; Smooth muscle myosin heavy chain 11; Myosin-11; SM1; SM2; SMHC; SMMHC

Database Links

Entrez Gene ID
4629
SwissProt
P35749

Additional Information

Clone
SM-M10
Chromosome Location
16p13.11
Mol. Weight of Antigen
205kDa (MHC-1) and 200kDa (MHC-2)

Related Antibodies

View All MYH11 Antibodies

Functions

  • Muscle contraction.

Key References

  • Lazard D, et al. Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):999-1003. doi: 10.1073/pnas.90.3.999. PMID: 8430113; PMCID: PMC45798.
  • N.P. Wang, at al. Applied Immunohistochemistry 1997;5(3):141-151.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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