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Printable Datasheet

SPARC / Osteonectin Antibody [OSTN/3755]

In Stock
Catalog Number Formulation Size Price
6678-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6678-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6678-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Western blot analysis of JEG-3 cell lysate using SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Western blot analysis of JEG-3 cell lysate using SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC / Osteonectin Mouse Monoclonal Antibody (OSTN/3755).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Endothelial Cell Marker, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
SPARC
Positive Control
Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.
Immunogen
Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
Alternate Names
SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, Basement membrane protein 40; BM40; Cysteine rich protein; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine (SPARC)

Database Links

Entrez Gene ID
6678
SwissProt
P09486

Additional Information

Clone
OSTN/3755
Chromosome Location
5q33.1
Mol. Weight of Antigen
38kDa

Related Antibodies

View All SPARC Antibodies

Functions

  • Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Key References

  • Galv n, J.A., et al. 2019. Immunohistochemical analysis of the expression of cancer-associated fibroblast markers in esophageal cancer with and without neoadjuvant therapy. Virchows Arch. E-published.
  • Yan, L., et al. 2017. Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions. Int. J. Mol. Med. 40: 1253-1260.

PubMed Links

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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