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Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody [rSPTA1/1832]

In Stock
Catalog Number Formulation Size Price
6708-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6708-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6708-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 2

SDS-PAGE Analysis Purified Spectrin alpha 1 Mouse Recombinant Monoclonal (rSPTA1/1832). Confirmation of Purity and Integrity of Antibody.

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 3

Western Blot Analysis of human K562 cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 4

Western Blot Analysis of human HeLa cell lysate using Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 5

Formalin-fixed, paraffin-embedded human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1832).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTA1
Positive Control
Jurkat cells. Placenta or Tonsil., K562. HeLa
Immunogen
Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)
Alternate Names
Spectrin alpha chain, erythrocytic 1, Erythroid alpha-spectrin, Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1

Database Links

Entrez Gene ID
6708
SwissProt
P02549

Additional Information

Clone
rSPTA1/1832
Chromosome Location
1q23.1
Mol. Weight of Antigen
240kDa

Related Antibodies

View All SPTA1 Antibodies

Functions

  • Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Key References

  • Speicher, D.W. 1986. J. Cell. Biochem. 30: 245-258.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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