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Spectrin beta III (SPTBN2) Antibody [SPTBN2/1584]

In Stock
Catalog Number Formulation Size Price
6712-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6712-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6712-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Spectrin beta III (SPTBN2) Antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Spectrin beta III (SPTBN2) Antibody - Image 2

Formalin-fixed, paraffin-embedded Rat Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Spectrin beta III (SPTBN2) Antibody - Image 3

Formalin-fixed, paraffin-embedded Mouse Kidney stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Spectrin beta III (SPTBN2) Antibody - Image 4

Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Spectrin beta III with Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1778) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Spectrin beta III (SPTBN2) Antibody - Image 5

Western Blot Analysis of HeLa cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Spectrin beta III (SPTBN2) Antibody - Image 6

SDS-PAGE Analysis Purified Spectrin beta III Monoclonal Antibody (SPTBN2/1584). Confirmation of Integrity and Purity of Antibody.

Spectrin beta III (SPTBN2) Antibody - Image 7

Western Blot Analysis of PANC-1 cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Rat
Research Areas
Developmental Biology, Immunology, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTBN2
Positive Control
HeLa cells. Pancreas or Liver., PANC-1
Immunogen
Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)
Alternate Names
Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Spinocerebellar ataxia 5 protein, Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

Database Links

Entrez Gene ID
6712
SwissProt
O15020

Additional Information

Clone
SPTBN2/1584
Chromosome Location
11q13.2
Mol. Weight of Antigen
246kDa

Related Antibodies

View All SPTBN2 Antibodies

Functions

  • Probably plays an important role in neuronal membrane skeleton.

Key References

  • Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. J. Cell. Biochem. 30: 245-258.
  • Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. Cytoskeleton 12: 225-247.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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