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SUMO-1 Antibody [SPM571]

In Stock
Catalog Number Formulation Size Price
7341-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7341-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7341-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO-1 Monoclonal Antibody (SPM571)

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO-1 Monoclonal Antibody (SPM571)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Infectious Disease, Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Nucleus, Nucleus membrane, Nucleus speckle, PML body
Gene Name
SUMO1
Positive Control
Breast carcinoma.
Immunogen
Recombinant human SUMO1 protein
Alternate Names
Small ubiquitin-related modifier 1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GAP-modifying protein 1; GMP1; OFC10; PIC1; SENP2; Sentrin 1; Small ubiquitin-related modifier 1; SMT3; SMT3 suppressor of mif two 3 homolog 1; SMT3C; SMT3H3; Ubiquitin homology domain protein PIC1; Ubiquitin Like 1; Ubiquitin like protein UBL1

Database Links

Entrez Gene ID
7341
SwissProt
P63165

Additional Information

Clone
SPM571
Chromosome Location
2q33.1
Mol. Weight of Antigen
11.5kDa (Monomer); 90kDa (Heteromer)

Related Antibodies

View All SUMO1 Antibodies

Functions

  • Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).

Key References

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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