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TCF4 (Transcription Factor 4) Antibody [TCF4/2252]

In Stock
Catalog Number Formulation Size Price
6925-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6925-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6925-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') – a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology, Colon Cancer
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Nucleus
Gene Name
TCF4
Positive Control
colon or tonsil., HeLa or HepG2 cells. Human bladder
Immunogen
Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)
Alternate Names
Transcription factor 4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, bHLHb19; Class B basic helix-loop-helix protein 19; Immunoglobulin transcription factor 2; ITF-2; PTHS; SEF2; SL3-3 enhancer factor 2; TCF4; Transcription factor 4

Database Links

Entrez Gene ID
6925
SwissProt
P15884

Additional Information

Clone
TCF4/2252
Chromosome Location
18q21.2
Mol. Weight of Antigen
60kDa

Related Antibodies

View All TCF4 Antibodies

Functions

  • Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

Key References

  • Van de Wetering, M., et al. 1991. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. EMBO J. 10: 123-132.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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