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TCF7 Antibody [TCF7/7631]

In Stock
Catalog Number Formulation Size Price
6932-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6932-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6932-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified TCF7 Mouse Monoclonal Antibody (TCF7/7631). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified TCF7 Mouse Monoclonal Antibody (TCF7/7631). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human lymph node stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lymph node stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Developmental Biology, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Membrane.
Gene Name
TCF7
Positive Control
adrenal gland or colon.|Human spleen|lung
Immunogen
Recombinant fragment (around aa200-400) of human TCF7 protein (exact sequence is proprietary)
Alternate Names
Transcription factor 7, T-cell-specific transcription factor 1, Transcription Factor 7 (T-Cell Specific, HMG-Box); T-Cell-Specific Transcription Factor 1; T-Cell Factor 7

Database Links

Entrez Gene ID
6932
SwissProt
P36402

Additional Information

Clone
TCF7/7631
Chromosome Location
5q31.1
Mol. Weight of Antigen
41.6kDa

Related Antibodies

View All TCF7 Antibodies

Functions

  • Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

Key References

  • Van de Wetering, M., et al. 1992. The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization. J. Biol. Chem. 267: 8530-8536.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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