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TFPI2 (Marker of Ovarian Carcinoma) Antibody [TFPI2/13027]

In Stock
Catalog Number Formulation Size Price
7980-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7980-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7980-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
TFPI2 Antibody in Western Blot (WB)

TFPI2 Antibody in Western Blot (WB)

Western blot analysis of human Placenta tissue lysate using TFPI2 Mouse Monoclonal Antibody (TFPI2/13027).

TFPI2 Antibody in Immunohistochemistry (IHC (P))

TFPI2 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human placenta stained with TFPI2 Mouse Monoclonal Antibody (TFPI2/13027). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The extrinsic pathway of blood coagulation is initiated by contact of plasma factor VII with tissue factor, a cellular membrane glycoprotein that normally is segregated from the bloodstream but can be exposed after tissue injury or newly synthesized in endothelial cells or leukocytes after stimulation by endotoxin and cytokines. Inhibition of Factor VIIa tissue factor activity requires a plasma component (tissue factor pathway inhibitor (TFPI), lipoprotein-associated coagulation inhibitor (LACI) or extrinsic pathway inhibitor (EPI)) and factor Xa. TFPI directly inhibits factor Xa, and, in an Xa-dependent fashion, also inhibits the Factor VIIa tissue factor catalytic complex. TFPI is a multivalent, Kunitz-type proteinase inhibitor that circulates in association with plasma lipoproteins VLDL, LDL, and HDL. TFPI-2 (also known as placental protein 5) is a related glycoprotein that was originally isolated from human placenta.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Cell Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
TFPI2
Positive Control
Human placenta.
Immunogen
Recombinant fragment (around aa100-235) of human TFPI2 protein (exact sequence is proprietary)
Alternate Names
tissue factor pathway inhibitor 2, Placental protein 5 antibody; PP5 antibody; REF1 antibody; Retinal pigment epithelium cell factor 1 antibody; TFPI 2 antibody; TFPI-2 antibody; TFPI2 antibody; TFPI2 PEN antibody; TFPI2_HUMAN antibody; Tissue factor pathway inhibitor 2 antibody, PP5; REF1; TFPI-2

Database Links

Entrez Gene ID
7980
SwissProt
P48307

Additional Information

Clone
TFPI2/13027
Chromosome Location
7q21.3
Mol. Weight of Antigen
27-32kDa

Related Antibodies

View All TFPI2 Antibodies

Functions

  • May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Key References

  • Zhao D, Qiao J, He H, Song J, Zhao S, Yu J. TFPI2 suppresses breast cancer progression through inhibiting TWIST-integrin α5 pathway. Mol Med. 2020;26(1):27. Published 2020 Apr 5. doi:10.1186/s10020-020-00158-2
  • Yan W, Han Q, Gong L, et al. MBD3 promotes hepatocellular carcinoma progression and metastasis through negative regulation of tumour suppressor TFPI2. Br J Cancer. 2022;127(4):612-623. doi:10.1038/s41416-022-01831-5

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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