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Recombinant TNF-alpha (Tumor Necrosis Factor alpha) Antibody [TNF/1500R]

In Stock
Catalog Number Formulation Size Price
7124-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7124-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7124-RBM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

Formalin-fixed, paraffin-embedded human Pancreas stained with TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

Formalin-fixed, paraffin-embedded human Pancreas stained with TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

SDS-PAGE Analysis of Purified TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

SDS-PAGE Analysis of Purified TNF alpha Rabbit Recombinant Monoclonal Antibody (TNF/1500R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Apoptosis, Autophagy, Cardiovascular, Developmental Biology, Immunology, AKT Signaling, Colon Cancer, Cytokine Signaling, Hematopoietic Stem Cells, MAPK Signaling, Neuroinflammation, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Membrane, Secreted
Gene Name
TNF
Positive Control
HeLa, HL-60 or A431 cells. Pancreas or Histiocytoma.
Immunogen
Recombinant full-length human TNF-alpha protein
Alternate Names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Database Links

Entrez Gene ID
7124
SwissProt
P01375

Additional Information

Clone
TNF/1500R
Chromosome Location
6p21.33
Mol. Weight of Antigen
17kDa

Related Antibodies

View All TNF Antibodies

Functions

  • Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).
  • The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Key References

  • Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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