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Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody [rTOP2A/8339]

In Stock
Catalog Number Formulation Size Price
7153-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7153-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7153-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/8339). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/8339). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a 170kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with Topoisomerase II β or I. Topo II α plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II α is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus.
Gene Name
TOP2A
Positive Control
HeLa or Jurkat cells. Human lymph node or tonsil.
Immunogen
Recombinant human Topoisomerase II alpha fragment (aa1300-1500) (exact sequence is proprietary)
Alternate Names
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A

Database Links

Entrez Gene ID
7153
SwissProt
P11388

Additional Information

Clone
rTOP2A/8339
Chromosome Location
17q21.2
Mol. Weight of Antigen
170kDa

Related Antibodies

View All TOP2A Antibodies

Functions

  • Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

Key References

  • Chung, T.D., et al. 1989. Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. Proc. Natl. Acad. Sci USA 86: 9431-9435.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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