Free Shipping in the U.S. for orders over $1000.  Shop Now>>

TRIM27 Antibody [PCRP-TRIM27-1B3]

In Stock
Catalog Number Formulation Size Price
5987-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5987-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5987-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2a /
Cellular Localization
Cytoplasm, Early endosome, Nucleus, PML body
Gene Name
Positive Control
HeLa, U87 or Raji cells.
Immunogen
Recombinant full-length human TRIM27 protein
Alternate Names
Zinc finger protein RFP, RING finger protein 76, RING-type E3 ubiquitin transferase TRIM27, Ret finger protein, Tripartite motif-containing protein 27, AW538890; DAAP-182E11.3; MGC189472; Ret finger protein; RFP transforming protein; Rfp1; RING finger protein 76; RNF76; Tripartite motif-containing protein 27; Zinc finger protein RFP

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-TRIM27-1B3
Chromosome Location
6p22.1
Mol. Weight of Antigen
58.49kDa

Functions

  • E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.

Key References

  • Zhang Y et al. TRIM27 functions as an oncogene by activating epithelial-mesenchymal transition and p-AKT in colorectal cancer. Int J Oncol 53:620-632 (2018).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “TRIM27 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK