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Tyrosinase (Melanoma Marker) Antibody [OCA1/812]

In Stock
Catalog Number Formulation Size Price
7299-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7299-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7299-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Tyrosinase (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded melanoma stained with Tyrosinase Monoclonal Antibody (OCA1/812).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Melanosome, Melanosome membrane
Gene Name
TYR
Positive Control
SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.
Immunogen
Recombinant human TYR protein
Alternate Names
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

Database Links

Entrez Gene ID
7299
SwissProt
P14679

Additional Information

Clone
OCA1/812
Chromosome Location
11q14.3
Mol. Weight of Antigen
70-80kDa

Related Antibodies

View All TYR Antibodies

Functions

  • This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (By similarity). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (By similarity).

Key References

  • Korner, A., et al. 1982. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. Science 217: 1163-1165.
  • Hearing, V.J., et al. 1987. Mammalian tyrosinase the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19: 1141-1147.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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