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Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody [TYRP1/8313R]

In Stock
Catalog Number Formulation Size Price
7306-RBM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7306-RBM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7306-RBM15-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human melanoma stained with TYRP1 Rabbit Recombinant Monoclonal Antibody (TYRP1/8313R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified TYRP1 Rabbit Recombinant Monoclonal Antibody (TYRP1/8313R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. �Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
TYRP1
Positive Control
SK-MEL-19|SK-MEL-23|SK-MEL-30 or SK-MEL-37 cells. Human melanoma.
Immunogen
Recombinant fragment (around aa200-400) of human TYRP1 protein (exact sequence is proprietary)
Alternate Names
5, 6-dihydroxyindole-2-carboxylic acid oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, 5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75, Tyrosinase-related protein 1 (TYRP1), TYRRP

Database Links

Entrez Gene ID
7306
SwissProt
P17643

Additional Information

Clone
TYRP1/8313R
Chromosome Location
9p23
Mol. Weight of Antigen
75kDa

Related Antibodies

View All TYRP1 Antibodies

Functions

  • Plays a role in melanin biosynthesis (PubMed:22556244, PubMed:16704458, PubMed:23504663). Catalyzes the oxidation of 5, 6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5, 6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+) (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:22556244, PubMed:16704458). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).

Key References

  • Orlow, S.J., et al. 1994. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex. J. Invest. Dermatol. 103: 196-201.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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