Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody [VEGFA/7758R]

In Stock
Catalog Number Formulation Size Price
7422-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7422-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7422-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7758R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7758R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7758R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7758R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, BBB VCAM-1 Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Infectious Disease, Neural Stem Cells, Signal Transduction, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm. Cell surface. Extracellular (secreted).
Gene Name
VEGFA
Positive Control
breast or ovarian carcinomas.|Tumor cells in astrocytomas
Immunogen
Recombinant fragment (around aa1-200) of human VEGFA protein (exact sequence is proprietary)
Alternate Names
Vascular endothelial growth factor A, long form | N-VEGF | VEGFA, Vascular permeability factor, MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA

Database Links

Entrez Gene ID
7422
SwissProt
P15692

Additional Information

Clone
VEGFA/7758R
Chromosome Location
6p12
Mol. Weight of Antigen
19-22kDa (reducing) and 38kDa-44kDa (non-reducing)

Related Antibodies

View All VEGFA Antibodies

Functions

  • Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).
  • Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437).
  • Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Key References

  • Tischer, E., et al. 1991. The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J. Biol. Chem. 266: 11947-11954.
  • Berse, B., et al. 1992. Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages and tumors. Mol. Biol. Cell 3: 211-220.
  • Folkman, J., et al. 1989. Induction of angiogenesis during the transition from hyperplasia to neoplasia. Nature 339: 58-61.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales