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Vimentin (Mesenchymal Cell Marker) Antibody [LN-6]

In Stock
Catalog Number Formulation Size Price
7431-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7431-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7431-MSM2-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Cat, Cow, Human, Mouse, Pig, Rabbit, Rat, Sheep
Research Areas
Apoptosis, Immunology, Cytokine Signaling, Neural Stem Cells, Ovarian Cancer, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Cytoskeleton, Nucleus matrix
Gene Name
VIM
Positive Control
Jurkat cells, Melanomas., Sarcomas
Immunogen
Human thymic nuclear extract
Alternate Names
Vimentin, VIM

Database Links

Entrez Gene ID
7431
SwissProt
P08670

Additional Information

Clone
LN-6
Chromosome Location
10p12.33
Mol. Weight of Antigen
57-60kDa

Related Antibodies

View All VIM Antibodies

Functions

  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
  • Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Key References

  • Stathopoulos, E., et al., J. Histochem. Cytochem. 37, 1363 (1989).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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