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Vinculin (Marker of Age-related Macular Degeneration) Antibody [VCL/2573]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
7414-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7414-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7414-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin each contain Actin binding sites. Reportedly, vinculin is a potential plasma biomarker for Age-related Macular Degeneration (AMD). The early detection of AMD using novel plasma biomarkers with genetic modeling may enable timely treatment and vision preservation in the elderly.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Focal adhesion, Sarcolemma
Gene Name
VCL
Positive Control
Human Bladder and Testis.
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)
Alternate Names
Vinculin, Metavinculin, CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; VCL; VINC

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
VCL/2573
Chromosome Location
10q22.2
Mol. Weight of Antigen
117kDa

Functions

  • Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Key References

  • Kim H-J, et al. Identication of vinculin as a potential plasma marker for age-related macular degeneration. Invest Ophthalmol Vis Sci. 2014;55:7166-7176.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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