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Recombinant YBX1 / Y-box Binding Protein 1 / YB-1 (Tumor Biomarker) Antibody [rYBX1/2430]

In Stock
Catalog Number Formulation Size Price
4904-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4904-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4904-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YBX1 Mouse Recombinant Monoclonal Antibody (rYBX1/2430).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YBX1 Mouse Recombinant Monoclonal Antibody (rYBX1/2430).

SDS-PAGE Analysis of Purified YBX1 Mouse Recombinant Monoclonal Antibody (rYBX1/2430). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified YBX1 Mouse Recombinant Monoclonal Antibody (rYBX1/2430). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients' prognosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Cytokine Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytoplasmic granule, Extracellular exosome, Nucleus, Secreted
Gene Name
YBX1
Positive Control
Breast or lung carcinoma., HEK293 or PC12 cells. Prostate, MCF-7
Immunogen
Recombinant human full-length YBX1 protein
Alternate Names
Y-box-binding protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B, Enhancer factor I subunit A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1

Database Links

Entrez Gene ID
4904
SwissProt
P67809

Additional Information

Clone
rYBX1/2430
Chromosome Location
1p34.2
Mol. Weight of Antigen
36kDa

Related Antibodies

View All YBX1 Antibodies

Functions

  • DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969). Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969). Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969). Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303). Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095). Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095). Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925). Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611). Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583). Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551). Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed:14718551). The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673).

Key References

  • Janz M., et al. Int J Cancer. 2002, 97 (3): 278-282.
  • Gimenez-Bonafe P, et al. Prostate. 2004, 59 (3): 337-349.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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