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YBX3 (Transcription Factor) Antibody [PCRP-YBX3-2D12]

In Stock
Catalog Number Formulation Size Price
8531-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8531-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8531-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus. Cytoplasm.
Gene Name
Positive Control
HeLa or U87 cells. Human skeletal muscle|testis or bladder carcinoma.
Immunogen
Recombinant full-length human YBX3 protein
Alternate Names
Y-box-binding protein 3, Cold shock domain-containing protein A, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, Y-Box Binding Protein; ZONAB; CSDA1; CSDA; Single-Strand DNA-Binding Protein NF-GMB; Cold Shock Domain-Containing Protein A; Cold-Shock Domain Containing A1; Y-Box-Binding Protein; DNA-Binding Protein; DbpA; DBPA; ZO-1-Associated Nucleic Acid-Binding Protein; Cold Shock Domain Protein A; Cold-Shock Domain Protein A

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-YBX3-2D12
Chromosome Location
12p13.2
Mol. Weight of Antigen
40.09kDa

Functions

  • Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full-length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression (By similarity).

Key References

  • Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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