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ZFYVE28 (Zinc Finger FYVE-type containing 28) Antibody [LST2/2426]

In Stock
Catalog Number Formulation Size Price
57732-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
57732-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
57732-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

ZFYVE28 belongs to the lst-2 family. It contains 1 FYVE-type zinc finger. The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not mono-ubiquitinated at Lys-87. Mono-ubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. ZFYVE28 is a negative regulator of epidermal growth factor receptor (EGFR) signaling. It acts by promoting EGFR degradation in endosomes when not mono-ubiquitinated. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Cytosol
Gene Name
Positive Control
Brain, Colon, Kidney or Stomach., PC3 cells. Testis
Immunogen
Recombinant full-length human LST2/ ZFYVE28 protein
Alternate Names
Lateral signaling target protein 2 homolog, Zinc finger FYVE domain-containing protein 28, Lateral signaling target protein 2 homolog; LST2; LYST2; Zinc finger FYVE domain-containing protein 28 (ZFYVE28)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
LST2/2426
Chromosome Location
4p16.3
Mol. Weight of Antigen
96kDa

Functions

  • Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated.

Key References

  • Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling.Dev. Cell 16:687-698, 2009.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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