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ZNF488 Antibody [PCRP-ZNF488-2D8]

In Stock
Catalog Number Formulation Size Price
118738-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
118738-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
118738-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF488 is a 340 amino acid transcriptional regulator belonging to the Kruppel C2H2-type zinc finger protein family. ZNF488 localizes to the nucleus and contains two C2H2-type zinc fingers. ZNF488 is encoded by a gene located on chromosome 10, which contains a plethora of interesting genes and represents between 4 and 4.5 percent of the total DNA in cells. Jackson-Weiss, Cowden and Usher syndromes are a few diseases related to genes on chromosome 10.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2a /
Cellular Localization
Nucleus.
Gene Name
Positive Control
HeLa cells.
Immunogen
Recombinant fragment (around aa170-307) of human ZNF488
Alternate Names
Zinc finger protein 488, FLJ32104

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-ZNF488-2D8
Chromosome Location
10q11.22
Mol. Weight of Antigen
40kDa

Functions

  • Transcriptional repressor. Plays a role in oligodendrocyte differentiation, together with OLIG2. Mediates Notch signaling-activated formation of oligodendrocyte precursors. Promotes differentiation of adult neural stem progenitor cells (NSPCs) into mature oligodendrocytes and contributes to remyelination following nerve injury.

Key References

  • Blackshaw S, et al. Nature methods15.5 (2018 May): 330-338.
  • Statement for PublicationAll investigators should include an acknowledgment in the publications the benefit from the use of this product. Following statement is suggested: PCRP-ZNF488-2D8 was developed by JHU/CDI under Protein Capture Reagents Program of NICHD of the NIH.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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