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Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE) is a widely used technique for separating and analyzing proteins based on their molecular weight (MW) and a fundamental tool in the field of molecular biology and protein research. This protocol outlines the steps to prepare the samples and run a gel SDS-PAGE.
Western blotting, also known as immunoblotting, is a widely used technique for detecting and quantifying specific proteins in a complex mixture and validating antibodies. This protocol outlines the key steps involved in performing a Western blot and serves as a guide for researchers and customers of antibody products.
Flow cytometry is a powerful technique used for the quantitative analysis of cell populations and the detection of specific cancer and cell biomarkers. This protocol outlines the key steps involved in performing flow cytometry, highlighting the use of antibodies for cell surface or intracellular marker detection.
Immunohistochemistry (IHC) is a powerful technique for visualizing specific proteins in tissue sections, providing valuable insights for research and diagnostics. This protocol outlines the steps for tissue rehydration, antigen retrieval, and the IHC process, which often involves utilizing antibodies.
