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07 December, 2023 by Anshul (neobio)
Are you often overwhelmed by the challenge of finding reliable and specific monoclonal antibodies for your sophisticated experiments? The importance of anti-cyclin E antibodies in research is undebatable. An essential component in cell cycle progression, cyclin E plays a pivotal role in instigating DNA replication by interacting with and activating cyclin-dependent kinase 2 (Cdk2). This interaction is vital during the G1-phase of the cell cycle. Hence, inaccuracies or faults in this mechanism can often lead to serious health disorders.
Understanding the role of cyclin E in the cell cycle can be complex. So, to shed light on the process, an anti-cyclin E antibody is used. It detects the presence and measures the amount of cyclin E in a sample, revealing the intricate interplay between agents within the cell. It helps scientists to comprehend and study the mechanisms of cell cycle regulation, playing a crucial role in pushing the envelope in fields that include cancer research, genetics, and cell biology.
However, purchasing the perfect anti-cyclin E antibody can seem daunting. To unravel this complexity, we will shortly guide you through the nuances of riding the waves of advancements in biotechnology.
Here is a snippet of what to expect:
Our infographic on ‘Understanding Cyclin E’ gives a quick visual rundown of the functions and importance of the cyclin E protein, and the role anti-cyclin E antibodies play in advancing scientific understanding.
Now, let’s walk together through these sub-topics to make your journey of purchasing the best anti-cyclin E antibody in 2024 as smooth as possible.
One of the most reliable antibodies for detecting cyclin E in research is the Anti-Cyclin E Antibody (HE12). This antibody is a high-quality IgG1 κ mouse monoclonal antibody that specifically detects cyclin E from mouse, rat, or human origin. It’s versatile and can be used in various experimental techniques, including Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), and Immunohistochemistry (IHC)(P).
The Anti-Cyclin E Antibody (HE12) is one of the top choices for researchers due to its high specificity and sensitivity. It detects cyclin E as a doublet at 50 kDa and a single band at 42 kDa. This antibody has been extensively cited in scientific literature, with over 820 citations, testifying to its reliability and usefulness in various research scenarios.
It’s available in both conjugated and non-conjugated forms, allowing researchers flexibility in their experimental designs. Furthermore, the conjugated forms can be used with popular fluorescent dyes like Alexa Fluor® for easy visualization and analysis.
Many researchers have found the Anti-Cyclin E Antibody (HE12) to be highly effective and reliable. User reviews often highlight its high specificity, excellent signal, and broad reactivity across species.
While polyclonal antibodies are produced by different B cell lines and recognize multiple epitopes on the antigen, monoclonal antibodies are produced by a single B cell clone and recognize a single epitope. Recombinant monoclonal antibodies, on the other hand, are genetically engineered and offer the highest level of consistency and reproducibility.
The choice between polyclonal, monoclonal, and recombinant monoclonal antibodies depends on your specific research needs. If you require high specificity and consistency across batches, a monoclonal or recombinant monoclonal antibody like Anti-Cyclin E Antibody (HE12) would be most suitable.
Custom antibodies offer the advantage of being tailored to fit your specific research needs. They provide a higher degree of control over the antibody production process, allowing for the development of antibodies with unique specificities or affinities.
NeoBiotechnologies is at the forefront of custom antibody production. With over 1,000 highly validated, monospecific Rabbit Recombinant Monoclonal Antibodies, the company offers a wide range of options for researchers. As noted by the company’s founder and CEO, Dr. Atul K. Tandon, these antibodies are ideal for various applications such as Immunohistochemistry, Flow Cytometry, Western Blotting, or Immunofluorescence.
In conclusion, whether you’re looking for a reliable antibody like Anti-Cyclin E Antibody (HE12) or a custom solution, NeoBiotechnologies has you covered. Their antibodies are designed with the needs of the modern researcher in mind, offering high specificity, reliability, and versatility.
When it comes to purchasing an anti cyclin E antibody, there are several crucial factors to consider. Your choice of antibody can significantly impact the success of your research. Here’s what to keep in mind.
Specificity is the foremost consideration when selecting an antibody. An antibody that specifically targets Cyclin E in your chosen species (e.g., Human, Mouse, Rat) will ensure accurate and reliable results. ThermoFisher supplies a range of antibodies with verified specificity for Cyclin E.
Validation is another crucial factor. An antibody that’s been validated in experiments provides assurance of its effectiveness. For instance, antibodies verified by cell treatment, knockout, and knockdown have been scientifically confirmed to target Cyclin E.
Lastly, the quantity of antibody required will depend on the scale of your research. Most suppliers offer a range of quantities, from 25 µg to 500 µL, so it’s important to calculate your needs accurately to avoid wastage and extra costs.
When it comes to pricing, it’s essential to understand what the package includes. Some suppliers may offer extra services, such as custom antibody creation. For instance, NeoBiotechnologies has created over 18,000 custom antibodies to meet specific research needs.
It’s also important to take note of any restrictions or conditions attached to the product. Some antibodies, such as those offered by Cell Signaling Technologies, have specific usage terms, such as restrictions on third-party distribution or modification.
By carefully considering these factors, you can ensure that the anti cyclin E antibody you purchase is perfectly suited to your specific research needs.
In the next section, we will discuss the future of Anti-Cyclin E Antibodies in Biomedical Research and provide some final thoughts on choosing the best antibody for your needs.
As we move into the future, the role of anti cyclin E antibodies in biomedical research is only set to increase. With advancements in technology and a deeper understanding of cell cycle regulation, the potential for breakthrough discoveries in disease diagnosis and treatment is tremendous. Cyclin E, being a critical regulator of cell cycle progression, is a key player in many pathological conditions and therefore, the need for reliable and validated antibodies like those from NeoBiotechnologies is paramount.
The emergence of custom antibodies is another trend that is shaping the future of biomedical research. With more than 18,000 custom antibodies created so far by companies like NeoBiotechnologies, researchers can now have antibodies tailor-made for their specific targets, species, and applications. This customization allows for a higher level of specificity and accuracy in experimental outcomes, thus opening up new avenues of exploration and discovery.
Choosing the right anti cyclin E antibody for your research is a critical decision that can significantly impact your results. The quality and specificity of the antibody, its validation for your application, and the reliability of the supplier are all factors that should be carefully considered.
NeoBiotechnologies, with its wide range of highly validated, monospecific Rabbit Recombinant Monoclonal Antibodies, has proven to be a trusted partner for researchers around the world. Their commitment to quality and their ability to provide custom solutions make them a preferred choice for many.
The best antibody is not just the one that works, but the one that works best for your specific needs. By considering all these factors, you can ensure that you choose the anti cyclin E antibody that is perfectly suited to your research.
For more information on the range of antibodies offered by NeoBiotechnologies, you can visit their product page. For more specific details on Cyclin E antibodies, the Cyclin E antibody resource page provides comprehensive information.