Introduction

Is the hunt for reliable cyclin E antibodies for your research leaving you chasing your tail? You’re not alone. The pursuit for these essential tools is key as they play a pivotal role in cellular investigations, specifically cell cycling and DNA replication. Understanding the role of cyclin E is crucial to advance research and therapeutic applications, especially in potentially treating conditions like cancer where cell replication is often unchecked.

Brief Overview of Cyclin E and Its Role in Cell Cycle

Cyclin E, a fundamental cell cycle protein, is the driving force for progression through the G1-phase of the cell cycle and the initiation of DNA replication. It achieves this through its activation of its catalytic partner, the cyclin dependent kinase 2 (Cdk2). It’s this vital function that places cyclin E at the heart of many cellular studies.

Importance of Cyclin E Antibodies in Research

Employing cyclin E antibodies in research is critical to track and understand the workings of cyclin E. Information gleaned from such investigation could play an instrumental role in developing treatments for diseases where cell replication is abnormal. However, the quest for finding highly specific and validated antibodies can be a challenge for many researchers like yourself.

For a snapshot of key information about cyclin E:

• Role: Essential for progression through the G1-phase of the cell cycle and initiation of DNA replication
• Catalytic Partner: Activates Cyclin Dependent Kinase 2 (Cdk2)
• Importance in Research: Central to studies on cell cycling and replication; potential implications for cancer treatments
• Challenge: Finding highly specific and validated cyclin E antibodies

Together at NeoBiotechnologies, we manufacture highly validated, monospecific rabbit recombinant monoclonal antibodies, trusted by the world’s top institutions and researchers for precision and reliability. So buckle up and join us as we delve into ‘cyclin E antibodies’ to support you in making an informed decision for your research needs. Stay tuned as we spotlight the 5 best cyclin E antibodies, stripping back the science to deliver you clear comparisons and aid your decision making.

Understanding Cyclin E Antibodies

Role of Cyclin E Antibody in Detecting Cyclin E Protein

The primary role of a Cyclin E antibody is to identify and bind to the Cyclin E protein in biological samples. This interaction is crucial in various laboratory techniques such as Western Blotting (WB),  Immunofluorescence (IF), and Immunohistochemistry (IHC), where accurate detection of specific proteins like Cyclin E is required.

Cyclin E, together with Cyclin D and Cyclin C, represents a putative G1 cyclin due to its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary of the cell cycle. A key interaction of Cyclin E is with the transcription factor E2F, which is critical for the expression of several S phase-specific proteins.

The use of Cyclin E antibodies allows for the precise detection and study of these interactions and processes, contributing to our understanding of cell cycle regulation and related diseases, such as cancer.

Antibodies Tailored to Your Research Needs

We offer antibodies in two main forms: conjugated and non-conjugated. 

Conjugated antibodies have a reporter molecule attached to them, such as a fluorescent dye or an enzyme. This allows for direct visualization or detection of the target protein without the need for a secondary antibody.

On the other hand, non-conjugated antibodies do not have a reporter molecule attached. This means that a secondary antibody, which recognizes the primary antibody and carries a reporter molecule, is required for detection.

At NeoBiotechnologies, we offer a wide range of both conjugated and non-conjugated Cyclin E antibodies. Our expert, Dr. Atul K. Tandon, ensures that each antibody is developed, manufactured, and rigorously validated to meet the highest standards of specificity and reproducibility.

Spotlight on Anti-Cyclin E1 [CCNE1/2460]

Anti-Cyclin E1 Antibody [CCNE1/2460]: Features and Applications

The Anti-Cyclin E1 antibody [CCNE1/2460] is a mouse monoclonal antibody suitable for WB and IHC-P applications. It has been tested on the HuProt human protein array containing over 21,000 proteins and confirmed to be monospecific, so you can be certain in your results. It reacts with human species and has been used successfully in studying human colon carcinoma tissues. This antibody is shipped at 4°C, ensuring its stability during transport .

At NeoBiotechnologies, we manufacture over 1000 highly validated, monospecific Rabbit Recombinant Monoclonal Antibodies. These are ideal for a range of applications, including Immunohistochemistry, Flow Cytometry, Western Blotting, or Immunofluorescence. Our Cyclin E antibody is developed, manufactured, and rigorously validated by our team of scientists, ensuring its sensitivity and reliability in your research.

Another notable Cyclin E antibody is the Alexa Fluor® conjugated antibody. Alexa Fluor® is a range of high-performance fluorescent dyes that provide exceptionally bright, photostable conjugates. This antibody, in particular, is suitable for applications that require fluorescence detection, such as flow cytometry and fluorescence microscopy.

In conclusion, the choice of the best Cyclin E antibody will depend on your specific experimental needs, including the species you are studying, the detection method you plan to use, and the availability of resources.

How to Choose the Right Cyclin E Antibody for Your Research

Selecting the right cyclin E antibody for your research can make a significant difference in your results. With an array of options available, it can seem daunting to decide which one to choose. However, when you consider a few key factors, you can make a more informed decision that fits the needs of your experimental design.

Factors to Consider When Selecting a Cyclin E Antibody

1. Host species: The host species of an antibody is crucial for its accuracy and reliability in cancer research. It affects reactivity, risk of cross-reactivity, compatibility with experiments, and overall effectiveness in detecting the target protein. Researchers need to weigh these factors when choosing antibodies for their studies. NeoBiotechnologies offers both mouse and rabbit Anti-CCNE1 antibodies.

2. Species reactivity: The species reactivity of the cyclin E antibody is crucial. An ideal antibody should be able to detect cyclin E from the species you are studying. For instance, all of the Cyclin E antibodies we offer detect cyclin E of human origin.

3. Application suitability: Not all antibodies work well in all applications. Some are ideal for western blotting (WB), while others work better with immunohistochemistry (IHC) or immunofluorescence (IF). The cyclin E antibody you choose should be suitable for your intended application.

4. Conjugation: Depending on your specific experimental needs, you might need either a conjugated or non-conjugated antibody. For instance, if you plan to use fluorescence detection, a conjugated antibody like the CF® conjugates would be more appropriate. If you need an antibody conjugated to a specific
   
5. Specificity and Sensitivity: The antibody should be highly specific to cyclin E to avoid cross-reactivity with other proteins. Additionally, it should be sensitive enough to detect the protein at low concentrations.

Importance of Antibody Validation and Reproducibility

Validation is a critical process that confirms the antibody’s performance in a specific application. For instance, at NeoBiotechnologies, we ensure that our antibodies are rigorously validated by our team of scientists.

Reproducibility, on the other hand, ensures that the antibody yields consistent results across different experiments, laboratories, and over time. This is a critical aspect to consider, especially when planning for long-term projects.

In conclusion, choosing the right cyclin E antibody is crucial for the success of your research. By taking into consideration the factors mentioned above, and ensuring that your chosen antibody is well validated and offers reproducibility, you can make a well-informed decision. The right tools can significantly aid in advancing your scientific research.

Conclusion

Recap of the Top 3 Cyclin E Antibodies

Choosing the right cyclin E antibody for your research is a significant decision that can heavily impact your results. We’ve discussed several options, each with its own unique features and applications.

1. Anti-Cyclin E1 Antibody [CCNE1/2460]: This option offers high specificity and sensitivity, making it ideal for research that requires precise detection of cyclin E.

2. Recombinant Rabbit Anti-Cyclin E1 Antibody [CCNE1/4935R]: This is a recombinant rabbit antibody that has been validated to work in WB and IHC. It reacts in human tissues and cells.

3. Recombinant Mouse Anti-Cyclin E1 Antibody [rCCNE1/4936]: This is a recombinant mouse monoclonal antibody that has been validated for WB and IHC. It reacts in human tissues and cells.

The Role of Cyclin E Antibodies in Advancing Scientific Research

Cyclin E antibodies play a vital role in advancing scientific research. They are key tools in understanding the intricacies of the cell cycle, and their use has been pivotal in making breakthroughs in various fields including cancer research, reproductive biology, and developmental studies.

Using these antibodies, researchers can identify and study the role of cyclin E in cell cycle regulation, and how its dysregulation can lead to diseases such as cancer. For instance, cyclin E overexpression has been linked to the development of various cancers, making it a potential therapeutic target.

In conclusion, choosing the right cyclin E antibody is vital for your research. By considering factors such as the antibody’s specificity, sensitivity, and validation status, you can select a product that will best support your scientific investigation.

For more resources on antibodies, we encourage you to explore our monoclonal antibodies and our recombinant monoclonal antibodies pages.