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28 November, 2023 by Anshul (neobio)
More accurate and reliable research – Isn’t that what we all want? It cannot be achieved without a fundamental component: highly-validated monoclonal antibodies. As pioneers in the field, we at NeoBiotechnologies understand how the right tool can make all the difference. Driving us to produce superior tools are recombinant rabbit monoclonal antibodies.
Traditional mouse monoclonal antibodies have caveats. They often yield inconsistent results and have high background staining – a scientist’s nightmare. But what if we could do better? Replace these traditional monoclonals with something distinctly superior? Queue in the powerful alternative: Recombinant Rabbit Monoclonal Antibodies. They pave the way for more precise and reliable results, crucial in applications such as immunohistochemistry, flow cytometry, western blotting, and more.
Hence, our focus in this article is to introduce you to these high performers and compare the top 5 recombinant rabbit monoclonal antibodies in the market today. You might wonder – Why Rabbits? Well, the unique traits of rabbits and their diverse immune response make them an ideal host for producing these monoclonal antibodies.
Understanding these antibodies, their advantages, and the intricate production process can be a game-changer for your research, and we’re here to guide you every step of the way.
A Quick Snap:
– Recombinant rabbit monoclonal antibodies are a superior alternative to traditional monoclonal antibodies.
– They provide more accurate and reliable results.
– They are produced using recombinant technology, maximizing consistency, sensitivity, control, specificity, and scalability.
– Rabbits’ unique immunological traits make them the preferred host for antibody production.
When it comes to antibody production, scientists often turn to rabbits for several reasons. Let’s dive deeper into why that is the case.
While mouse monoclonal antibodies have been an invaluable tool in scientific research for many years, they do possess certain limitations. Notably, the genetic similarity between mice and humans can lead to reduced sensitivity to certain antigens. Additionally, inbreeding in mouse strains can decrease the diversity of immune responses, affecting the overall sensitivity of the produced antibodies.
Rabbits, on the other hand, boast unique traits that make them ideal for monoclonal antibody production. For one, rabbits exhibit a naturally heightened immune response compared to other laboratory animals. This means they can elicit robust responses to a wide variety of antigens, including small molecules and many natural and synthetic antigens, without the aid of carrier proteins.
Moreover, rabbits generate a diverse antibody repertoire due to their unusual genetics. This diversity is facilitated by somatic gene conversions and hypermutation mechanisms, allowing rabbits to mount a robust response even to low immunogenic compounds.
Also, unlike mice and rats, rabbit strains used in laboratories are outbred, enhancing the diversity of immune responses and the sensitivity of the antibodies produced.
Finally, rabbits have anatomical differences that are advantageous for the generation of monoclonal antibodies. For instance, B cells, critical for antibody production, are present in higher quantities in rabbits compared to mice and rats.
Rabbit monoclonal antibodies are superior to their mouse counterparts in several ways. For instance, rabbit antibodies have a longer third complementarity-determining region (CDR H3), often the site of the most significant contact with an antigen. This unique feature means rabbit monoclonals typically have a 10-100 times higher affinity than mouse monoclonals and yield better results in many assays.
Moreover, the evolutionary distance between rabbits and humans allows rabbits to be uniquely immunogenic to certain antigen epitopes which rodents are not. This means that the total pool of specific antibodies from rabbits is larger and more diverse, covering a broader range of epitopes.
In terms of stability, rabbit monoclonal antibodies boast a longer shelf life and overall stability due to extra disulfide bonds within their immunoglobulin heavy chain variable region.
All these factors make recombinant rabbit monoclonal antibodies an exceptional tool in research and development. At NeoBiotechnologies, we are proud to manufacture over 500 highly validated, monospecific Rabbit Recombinant Monoclonal Antibodies, offering precision and reliability that researchers can trust.
Next, we’ll delve into a comparison of the top 5 recombinant rabbit monoclonal antibodies. Stay tuned.
With a diverse portfolio of over 500 monospecific, recombinant rabbit monoclonal antibodies, we are confident in providing you the best for your research needs. Let’s take a closer look at the top 5 recombinant rabbit monoclonal antibodies and their unique benefits.
This recombinant rabbit monoclonal antibody is hosted in a rabbit and is highly reactive towards human cells. Its isotype, IgG, kappa, ensures high specificity and minimal cross-reactivity. The antibody is ideal for detecting the Rps23 gene, a significant gene involved in protein synthesis. Its price ranges from $219.00 to $499.00. Check it out here.
This antibody plays a crucial role in understanding the human ACE2 gene, which is highly relevant in COVID-19 research. It is hosted in a rabbit and exhibits high reactivity towards human cells. This recombinant rabbit monoclonal antibody is priced between $219.00 and $499.00. Find out more here.
This antibody is ideal for detecting the PGM5 gene, which plays a critical role in glucose metabolism. It can be used in various applications such as ELISA, Immunofluorescence, Immunohistochemistry, and Western Blot. Its price ranges from $219.00 to $499.00. Explore it here.
This recombinant rabbit monoclonal antibody is highly reactive towards human cells and is expected to show a broad species reactivity. It is especially beneficial for detecting the POMC gene, a precursor molecule for ACTH. It is priced between $219.00 and $499.00. Know more about it here.
This antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. It is priced between $219.00 and $499.00. Check it out here.
In conclusion, our recombinant rabbit monoclonal antibodies offer high specificity, reproducibility, and versatility, making them perfect for your research needs. Check out our complete list of recombinant rabbit monoclonal antibodies for more options.
After exploring the top recombinant rabbit monoclonal antibodies, let’s look at the validation and characterization process that ensures their quality and effectiveness.
We at NeoBiotechnologies take the validation of our recombinant rabbit monoclonal antibodies seriously. Every antibody undergoes rigorous testing to confirm its target specificity and functionality. We don’t stop at just one application; our antibodies are validated across multiple applications, ensuring their utility in a wide range of research contexts.
This in-depth validation process means you can trust our antibodies to deliver consistent, reliable results in your research, without the need for further optimization. Plus, with the availability of our extensive portfolio, you have plenty of options to choose from.
Our recombinant rabbit monoclonal antibodies aren’t just produced; they’re engineered with precision. We utilize cutting-edge techniques like CRISPR-Cas9 genome editing to enhance the specificity of our antibodies.
By making targeted modifications to the rabbit’s genome, we can encourage the production of B cells that generate highly specific antibodies against the desired antigen. This level of precision ensures that our antibodies bind with high affinity to their target, contributing to the accuracy of your research results.
In addition to specificity, reproducibility is another important feature of our recombinant rabbit monoclonal antibodies. Whether you’re performing Immunohistochemistry, Flow Cytometry, Western Blotting, or Immunofluorescence, you can expect consistent performance from our antibodies.
Reproducibility isn’t just about the antibody itself; it’s also about the systems in which the antibody is used. That’s why we validate our antibodies in multiple applications, ensuring they work reliably in the diverse experimental setups you might use in your research.
The validation and characterization of our recombinant rabbit monoclonal antibodies is a testament to our commitment to providing high-quality, reliable tools for research. Whether you’re exploring immunotherapy, studying cancer, or delving into other areas of research, we’re here to support you with the right antibodies for your needs.
When it comes to the production of our recombinant rabbit monoclonal antibodies, we strive for perfection. We know how crucial the accuracy and reliability of these antibodies are to your research. That’s why we’re constantly optimizing our manufacturing process, focusing on two key areas: culturing conditions and purification methods.
It’s a well-known fact that culturing conditions can significantly impact the quality of antibodies. For instance, a study published by Elsevier found that the quality of a recombinant rabbit monoclonal antibody can be improved by optimizing the transient expression conditions.
In our processes, we’ve found that culturing at lower temperatures, around 32°C, can result in a higher quality antibody with less heterogeneity. This reduction in protein heterogeneity is likely due to favorable effects on glycosylation and aggregation levels.
After production, the purification of the recombinant rabbit monoclonal antibody is a critical step. Different purification methods can lead to varying degrees of antibody quality. In our practice, we’ve found that a combination of ammonium sulfate fractionation and diethylaminoethyl (DEAE) ion exchange chromatography yields high-quality antibodies.
In contrast, purification methods such as Protein A/G affinity can sometimes lead to lower quality products. Our goal is to avoid any inferior charge variants that may result from non-specific reactions.
Our commitment to quality doesn’t stop at culturing and purification. We continually strive to enhance the quality of our recombinant rabbit monoclonal antibodies through further engineering. This can include tweaking our antibodies to reduce immunogenicity, increase binding affinity, or enrich manufacturing and storage capabilities.
Our recombinant antibody production process also minimizes the need for multiple animal immunizations, ensuring long-term consistency between product lots.
In conclusion, optimizing the production of recombinant rabbit monoclonal antibodies is a complex, multi-step process. But we believe it’s worth it. By continually refining our techniques and methods, we can ensure the production of superior antibodies that will deliver reliable, reproducible results in your research.
Just as the last note of a symphony leaves the audience anticipating the next performance, so too does our exploration of recombinant rabbit monoclonal antibodies leave us excited for the future. These antibodies have the potential to revolutionize disease treatment and play an instrumental role in research and development. And as we move forward, we anticipate continued evolution and improvements in this fascinating field.
Recombinant rabbit monoclonal antibodies are not just a tool for research; they could be the key to unlocking new treatments for numerous diseases. Their unique ability to recognize and bind to specific antigens with high affinity and specificity makes them ideal candidates for therapeutic interventions. For example, they could be used to target and neutralize harmful pathogens or to block specific biological pathways involved in disease progression.
But the potential of these antibodies doesn’t stop there. Because we can engineer them to bind to virtually any target, the possibilities for disease treatment are virtually limitless. The future holds exciting possibilities for the application of recombinant rabbit monoclonal antibodies in medicine.
In the context of research and development, recombinant rabbit monoclonal antibodies are invaluable. They are highly specific, incredibly versatile, and easy to produce in large quantities. This makes them ideal for a wide range of applications, from Immunohistochemistry and Flow Cytometry to Western Blotting and Immunofluorescence.
Our extensive portfolio of over 10,000+ monospecific, monoclonal antibodies, both recombinant and hybridoma, are developed, manufactured, and rigorously validated by our team of scientists. This ensures that you, as a research scientist, can rely on our antibodies for precision and consistency in your research.
The beauty of science is its constant evolution, and the field of recombinant rabbit monoclonal antibodies is no exception. With the advancement of technology and our ever-increasing understanding of the immune system, we continue to refine our methods and improve the quality of our antibodies.
From enhancing the culturing conditions to fine-tuning purification methods, we are committed to pushing the boundaries of what’s possible. And as we look to the future, we’re excited by the prospect of discovering new ways to enhance the performance of our recombinant rabbit monoclonal antibodies, ensuring that they remain the tool of choice for researchers and scientists around the globe.
In conclusion, the future of recombinant rabbit monoclonal antibodies is bright. Whether it’s unlocking new treatments for diseases or advancing scientific research, these antibodies have an important role to play. And here at NeoBiotechnologies, we’re proud to be at the forefront of this exciting field. As we continue to push the boundaries of what’s possible, we look forward to sharing our discoveries and advancements with you.
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