TRIM24 / TIF1 alpha Antibody [PCRP-TRIM24-1B12]

Catalog Number Formulation Size Price
8805-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
8805-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
8805-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

TIF1伪, also known as TRIM24, mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. TIF1伪 localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. TIF1伪 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. The TIF1伪 gene, which maps to human chromosome 7q33, encodes two alternatively spliced transcripts. However, the full length nature of one variant has not been determined. A TIF1伪 homolog (designated bonus) has been identified in Drosophila and is associated with several genes that are implicated in the ecdysone pathway, a nuclear hormone receptor pathway required throughout Drosophila development, suggesting a conserved functional role for the protein throughout the course of evolution.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Cytoplasm, Mitochondrion, Nucleus
Gene Name
Positive Control
HeLa or K562 cells.
Immunogen
Recombinant full-length human TRIM24 protein
Alternate Names
Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, Tripartite motif-containing protein 24, E3 ubiquitin protein ligase TRIM24; hTIF1; PTC6; RING finger protein 82; RNF82; TF1A; TIF1 alpha; Transcriptional intermediary factor 1 alpha; Tripartite motif-containing protein 24

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-TRIM24-1B12
Chromosome Location
7q33
Mol. Weight of Antigen
117kDa

Functions

  • Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed:24820418). Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity). Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed:32324863). Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed:33724611).

Key References

  • Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  • Fraser, R.A., et al. 1998. J. Biol. Chem. 273: 16199-16204.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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